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A head-to-head amplification resembling the TID structure described above was found in yeast following prolonged growth under selection ( Araya et al. 2010 ). This amplification arose between two short palindromes and was explained by a model in which a nascent leading single strand with a palindromic 3′ end anneals across the fork to initiate repair synthesis templated on the opposite sister chromosome ( Brewer et al. 2011 ). This replication extends away from the fork of origin and toward another fork moving away at the opposite end of a replication bubble. There it switches back to the original leading strand template with the aid of a second palindrome. Switches at both diverging forks produces a head-to-head dimeric circle, which can be extracted and integrated into the chromosome to yield a symmetrical TID. Fork interactions at replication bubbles were also proposed to explain duplication by unequal translocation in human cancer cell lines ( Howarth et al. 2011 ). The formation of TID duplications has also been explained using a template-switching model that does not restrict template switches to replication fork regions ( Hastings et al. 2009b ; Carvalho et al. 2011 ).

Duplication by Template Switching

In several models, joining of dissimilar sequences is attributed to replication template switching. Although these models can, in principle, explain the origin of TIDs with SJ sequences, they are mechanistically a bit tortuous and do little more than restate the features of the duplications they explain. One of the initial models (FoSTes) proposes that the 3′ end of a nascent Okazaki fragment anneals with the lagging strand template of a different replication fork on the opposite side of the gene being duplicated ( Gu et al. 2008 ). A subsequent model, called microhomology-mediated break-induced replication (MMBIR), builds on break-induced replication ( Clearance Wide Range Of Many Styles Finders Breezeblocks Wide Leg Jumpsuit Black Finders Keepers Online Cheap Authentic Exclusive Cheap Online Outlet Low Shipping Fee EHNwBa6
). This model suggests that a double-strand end is resected to produce a 3′-ended single-strand overhang that can prime replication at a distant point in duplex DNA without benefit of a strand invasion protein. This replication start juxtaposes sequence from the priming strand with that of the newly synthesized template complement. If such forks collapse, the extended 3′ end can reinitiate elsewhere in the genome and thus produce complex rearrangements. This illegitimate initiation is said to become more likely during growth inhibition because of repression of the enzymes responsible for homologous recombination ( Hastings et al. 2009b ). The fork made in this way is unstable and subject to collapse during subsequent replication. Each collapse releases a new extended single 3′ end that can repeat the priming at a new distant point. The extending 3′ end thus accumulates sequence blocks from multiple genome regions in both orientations. The process of RecA-independent replication initiation has been shown in vitro ( Sale Release Dates View Sale Online Gina kimono Blue Gilda amp; Pearl Discount Fashionable Fast Delivery Online ffqUm3fhQJ
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). These models may account for the complex rearrangements inferred to occur in some metazoan genomes ( Liu et al. 2011 , 2012 ), especially those thought to join sequences from different chromosomes. The models can also accommodate the TIDs found in bacteria and yeast.

Some time ago, Barbara McClintock suggested the BFB cycle as a way of forming of inversion duplications and amplifications during mitosis ( McClintock 1941 ). In her model, a chromosome breaks before replication and the broken ends of two copies fuse to generate a dicentric chromosome (see Fig. 7 ). At cell division, this dicentric breaks asymmetrically to form one chromosome with a terminal duplication and another with a corresponding deletion. The duplication-bearing chromosome lacks telomeres and replicates to form sisters that are subject to fusion and formation of another dicentric. Breakage then produces a chromosome with four copies of the repeat (two pairs of inverse-order repeats). Multiple mechanisms have been suggested to explain the breakage, fusion, and stabilization final product, but repetition of the BFB cycle continues to generate higher and higher copy number amplifications of inverse-order repeats.

Sets the repetition frequency of the ranging operation in Hertz. Upon entering the desired frequency the system will calculate the nearest frequency that it can handle according to the resolution of internal timers.

Sets the number of pulses to be used in multi-pulse averaging mode. In this mode, a sequence of rapid fire ranges are taken and then averaged to improve the accuracy of the measurement

Sets the system sensitivity. Larger values of THR represent higher sensitivity. The system may limit the maximum value of THR to prevent excessive false alarm rates based on settings made at the factory. Set to -1 for automatic threshold adjustments

Desired baud rate

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What type of RPM sensor is connected

RPM_SCALING: RPM scaling

Scaling factor between sensor reading and RPM.

RPM_MAX: Maximum RPM

Maximum RPM to report

RPM_MIN: Minimum RPM

Minimum RPM to report

Minimum data quality to be used

RPM_PIN: Input pin number Swim shorts Madeira Airstop red patterned Fedeli Discount Sale 6zf15oIX

Which pin to use

RPM2_PIN: RPM2 input pin number

Which pin to use

RSSI_TYPE: RSSI Type

Radio Receiver RSSI type. If your radio receiver supports RSSI of some kind, set it here, then set its associated RSSI_XXXXX parameters, if any.

RSSI_ANA_PIN: Receiver RSSI analog sensing pin Womens Easy Sleeveless Dress Bench Outlet Websites Cheap Sale Visit gbUMGnV

This selects an analog pin where the receiver RSSI voltage will be read.

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This is the voltage value that the radio receiver will put on the RSSI_ANA_PIN when the signal strength is the weakest. Since some radio receivers put out inverted values from what you might otherwise expect, this isn’t necessarily a lower value than RSSI_PIN_HIGH.

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This is the voltage value that the radio receiver will put on the RSSI_ANA_PIN when the signal strength is the strongest. Since some radio receivers put out inverted values from what you might otherwise expect, this isn’t necessarily a higher value than RSSI_PIN_LOW.

RSSI_CHANNEL: Receiver RSSI channel number

The channel number where RSSI will be output by the radio receiver (5 and above).

RSSI_CHAN_LOW: Receiver RSSI PWM low value

This is the PWM value in microseconds that the radio receiver will put on the RSSI_CHANNEL when the signal strength is the weakest. Since some radio receivers put out inverted values from what you might otherwise expect, this isn’t necessarily a lower value than RSSI_CHAN_HIGH.

RSSI_CHAN_HIGH: Receiver RSSI PWM high value

This is the PWM value in microseconds that the radio receiver will put on the RSSI_CHANNEL when the signal strength is the strongest. Since some radio receivers put out inverted values from what you might otherwise expect, this isn’t necessarily a higher value than RSSI_CHAN_LOW.

Set to non-zero to enable scheduler debug messages. When set to show “Slips” the scheduler will display a message whenever a scheduled task is delayed due to too much CPU load. When set to ShowOverruns the scheduled will display a message whenever a task takes longer than the limit promised in the task table.

Interviews were recorded by digital voice recorder and transcribed and translated into English soon afterwards, whilst retaining key words and phrases in Arabic. For accuracy, the two bilingual research specialists crosschecked several transcripts. The lead investigator read the interviews immediately and discussed any points for clarification and follow-up questions were developed. When possible, the lead investigator conducted interviews in English or in Arabic (with the assistance of the research specialists). Wherever possible contact with participants was maintained as is typical of in-depth ethnographic research to allow for follow-up and more informal discussions and observations. This approach allows for gradual accumulation of data and slow inductive analysis with further interviews and fieldwork interactions shaped by emerging understandings. Such an approach means developing rapport and gaining deeper insight into how miscarriage is understood in the wider social and cultural matrix. Amongst the pregnancy cohort twelve participants were interviewed one to two times, five were interviewed between six and nine occasions. In the miscarriage cohort seven participants were interviewed on three to six occasions and others were interviewed once to twice. The number of times a participant was interviewed was based on their interest and availability. To preserve anonymity, identifying participant details have been altered or omitted. Additional data included documents such as patient flyers and posters, detailed fieldnotes and fieldwork diaries.

Analysis of the qualitative data sets generated by the research was through a process of thematic coding and analysis. The basis of analysis was deep familiarity with the data and an ongoing, recursive process of reading, discussing and reflecting on the data as it is collected [ 42 , 43 ]. Through ongoing discussions and regular team meetings we reflected on developing understandings, and collected additional data as necessary. Emerging understandings and the identification of key knowledge gaps informed further interviews and fieldwork interactions. We identified themes and put them into categories, with higher order categories emerging in line with the constant comparative method [ 44 ]. The value of this process of data collection and analysis is that it yields in-depth, nuanced data from multiple perspectives, which captures the complexity of participant’s experiences and the networks of meaning within which they are embedded.

Quantitative data such as age, employment status, number of pregnancies, number of miscarriages, and number of children for all participants was extracted and entered into tables. Data was organised into categories, interrelated with the abstracted themes and then analyzed for identification of group patterns. Simple descriptive statistics were used to examine the patterns of socio-demographic data.

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